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Phenotypic analysis of T cells expanded using various culture media in the presence or absence of serum. T-cell activation using LymphoONE T-Cell Expansion Xeno-Free Medium in combination with anti-CD3 antibody, IL-2, and RetroNectin reagent results in enhanced T-cell expansion and yields higher proportions of naïve T cells relative to approaches employing IL-2 in combination with anti-CD3 and anti-CD28 antibodies.Īs demonstrated by the data below, expansion in LymphoONE medium also yields higher proportions of naïve T cells as compared to other commonly used media, with or without the addition of serum. By enabling robust expansion in the absence of human serum or plasma, LymphoONE medium is well-suited for immunotherapy research involving T cells. LymphoONE medium contains no proteins other than pharmaceutical-grade human serum albumin and recombinant human insulin, and provides superior performance relative to other commonly used xeno-free media.Ĭonventional culture systems typically require the addition of serum (or plasma) in order to achieve sufficient quantities of T cells for clinical research applications, but the availability of autologous serum varies, and it has been demonstrated that T cells expanded in the absence of serum can offer improved therapeutic performance (Janetzki et al. It has been optimized to enable efficient T-cell proliferation with or without the addition of human serum or plasma. LymphoONE T-Cell Expansion Xeno-Free Medium is specifically formulated for expansion and transduction of human T lymphocytes.